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Research Technician I or II

Research Technician I (job #3170926)
Research Technician II (job #3170931)
The Moon Lab at the Center for Immunology and Inflammatory Diseases (CIID) at Massachusetts General Hospital - Harvard Medical School is seeking a full-time Research Technician I or II to immediately assist in cellular immunology research related to the development of new coronavirus vaccines.
The laboratory studies basic CD4+ T cell biology through the use of advanced mouse and human models of adaptive immunity. See for details. The lab has recently begun a major program project with other labs in the Boston community to develop next generation pan-coronavirus vaccines. We are seeking a highly motivated, skilled, and exceptionally organized research technician to assist in a diverse range of duties related to all aspects of the lab, but the main responsibility will be to carry out assays to measure T cell function during immune responses to SARS-CoV-2 and other coronaviruses.
Job responsibilities will include:
- general lab maintenance
- minor oversight of mouse colony (no weaning or genotyping)
- basic mouse procedures (injections)
- mouse dissection and tissue preparation
- human blood and tissue processing
- tissue culture
- cellular assays including ELISpot and AIM (activation-induced marker)
- flow cytometry with peptide:MHC tetramers
- molecular assays including cDNA preparation for single cell RNA-seq analysis
The applicant must have a B.A. or B.S in a science-related discipline, preferably with some background in immunology. Experience in mouse handling, tissue culture, molecular biology, and especially flow cytometry is highly desired, but not required. The applicant must be extremely well organized, technically proficient, critically minded, and able to work independently. Salary is commensurate on relevant lab experience. Applicants must be US citizens or permanent residents (department will not sponsor visas).
Interested applicants should submit a resume, contact information for 2 references, and a short description of relevant work experience and long-term career goals to Dr. James Moon (, or apply online through the MGH Careers website: