Technical Research Assistant II Dinulescu Research Lab BWH
The Dinulescu Laboratory at Brigham and Women’s Hospital is seeking a highly motivated and hard-working college graduate to join our lab. Our research broadly centers around translational oncology: ultra-sensitive tumor biomarkers in liquid biopsies for cancer screening; imaging technologies for the early detection of cancer; precision medicine; and improving the safety and efficacy profiles of immunotherapies in women’s cancers. We focus on analyzing patient samples collected from immunotherapy clinical trials and using clinically relevant animal models/organoid tumor systems to improve patient responses and clinical outcomes. The successful applicant will be able to work independently under the general supervision of the Principal Investigator and in accordance with established hospital policies and procedures. The applicant will be expected to execute experiments involving in vitro and in vivo protocols, advanced molecular genetics, and cellular biology techniques. Additionally, they will have the opportunity to collaborate with researchers across Boston from Massachusetts General Hospital, Dana-Farber Cancer Institute, and the Whitehead Institute at MIT.
PRINCIPAL DUTIES AND RESPONSIBILITIES:
- Independently conducts molecular biology procedures, such as DNA extraction, conventional PCR and real time PCR, western blotting, and MTT assays.
- Maintains tissue cultures and manipulates cell lines in a variety of experimental conditions. Monitors cells/cultures for viability and contamination. Perform cell viability MTT assays using various chemotherapy compounds and molecularly targeted therapies. Performs lentiviral and retroviral infection, plasmid transfection, and immunoblotting of tumor cells and controls.
- Performs in vivo work using animal models of cancer and precursor lesions of the disease, which will involve intraperitoneal and intravenous inoculation of tumor cells and administration of therapeutics, serial blood collection via the mandibular or ocular vein, blood collection via cardiac puncture and tumor collection at necropsy, tumor imaging, survival surgery for orthotopic tumor inoculation, and other specialized techniques.
- Performs immunohistochemistry, immunofluorescence, and FACS protocols for immune-oncology, biomarker screening, and precision medicine.
- Contributes to the set-up and optimization of murine and patient-derived 3D organoid tumor systems and the testing of immunotherapies in organoids.
- Calculates and analyzes data using statistical techniques and participates in the writing of manuscripts submitted for publication and grant applications.
- Discusses progress of work regularly with the Principal Investigator (PI).
- Maintains tissue culture and molecular biology supplies and reagents and helps order new supplies and minor equipment.
- Coordinates laboratory compliance with institutional policies and procedures in the areas of safety, environmental, and infection control.
- Assists in the orientation and training of new laboratory staff, COOP/undergraduate students, and interns.
- Performs all other duties and responsibilities as directed, including both research and administrative duties.
- Participates in the design and modification/troubleshooting of research protocols.
- Develops research methodologies within the parameters of experimental protocols and research objectives.
- Composes and may present sections of research reports and manuscripts.
QUALIFICATIONS:
Bachelor of Science or Bachelor of Arts in a biological science.
At least 1-2 years of experience with animal models of disease (intraperitoneal and intravenous inoculation of tumor cells and therapeutics, serial blood collection via the mandibular or ocular vein, blood collection via cardiac puncture at necropsy, survival surgery for orthotopic tumor inoculation), tissue culture techniques, immunohistochemistry, immunofluorescence, FACS, PCR. Knowledge of immuno-oncology and organoid tumor systems also helpful but not required.
SKILLS/ ABILITIES/ COMPETENCIES REQUIRED:
Sound analytical and organizational skills.
High degree of computer literacy.
Careful attention to detail.
Highly motivated and able to performs experiments independently under the guidance of the PI.
Able to conduct in vitro and in vivo experiments.
Excellent oral and written communication skills.
Must have sound interpersonal skills.
EEO Statement
Brigham and Women’s Hospital is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, sex, color, religion, national origin, sexual orientation, protected veteran status, or on the basis of disability.
Primary Location MA-Boston-BWH Longwood Medical Area
Work Locations BWH Longwood Medical Area 75 Francis Street Boston 02115
Job Technician/Technologist/Scientist
Organization Brigham & Women's Hospital(BWH)
Schedule Full-time
Standard Hours 40
Shift Day Job
Employee Status Regular
Recruiting Department BWH Pathology
Job Posting Apr 29, 2022