Associate Researcher (Drug Discovery & Development) - Seaver Autism Center
The Seaver Autism Center is looking for an Associate Researcher to support the Drug Discovery and Development Program. This is an entry-level laboratory research position, responsible for conduct of routine and standardized experiments under the supervision of the Principal Investigator or a senior researcher. This individual assists in the interpretation of research outcomes and may be responsible for lab and clerical support.
Essential Duties:
- Under direct supervision, conduct routine and standardized experiments using appropriate research techniques specific to subject matter of the research project. Primary focus of this role will be cellular assays to elucidate function of autism-related genes.
- Performs assays to support research studies.
- Maintains laboratory environment and assists in the upkeep of appropriate inventory
- Assists in the collection, analysis and review of experimental data for publication and presentation.
- Minimum two-year commitment preferred due to a substantial training effort associated with the position
Lab Responsibilities:
- Manual cell culture of cell lines including (but not limited to) human embryonic/induced pluripotent stem cells and differentiated cell types (neurons), mouse glia, HEKs.
- Perform automation protocols for cell expansion and differentiation, including iPSC expansion and QC, and neuronal differentiations on medium and high throughput platforms.
- Characterize iPSC and differentiated cells using immunofluorescence, quantitative PCR and Western blotting
- Perform quality control on iPSC bank by performing mycoplasma test, genotyping, and preparing samples for karyotyping.
- General molecular and biochemistry protocols.
- Follow established SOPs and work with senior scientists for protocol refining and troubleshooting
- Collaborate with team members to ensure experiments are well-planned and executed
- Process, organize, and summarize data using electronic/written lab notebook detailing experimental steps and results
- Analyze and present data at group meetings, participate in company internal discussions
Requirements:
- A minimum of bachelors in a relevant discipline is required
- Experience with human/mammalian cell culture
- A strong interest in stem cell and neurosciences
- 1-2 years of research/laboratory experience preferred
- Excellent oral and written communication skills, teamwork, enthusiasm, and a flexible work schedule are essential
- Hands-on experience with maintenance of pluripotent stem cells and differentiation protocols
- Prior experience performing basic molecular biology techniques such as qPCR, cloning, and immunofluorescence
- Motivation to learn new tools and technologies, attention to detail, and critical thinking