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Research Technician

Research Technician I (job #3223115)
The Moon Lab at the Center for Immunology and Inflammatory Diseases (CIID) at Massachusetts General Hospital - Harvard Medical School is seeking a full-time Research Technician I to assist in cellular immunology research.
The laboratory studies basic CD4+ T cell biology through the use of advanced mouse and human models of adaptive immunity. See for details. One major project involves the study of how autoreactive T cells initially become activated during the development of autoimmunity in mice. Another project investigates the biology of T cells that recognize commensal bacteria in the gastrointestinal tract. Additional projects include studies of antibody-induced regulatory T cell (Treg) activation, resident memory CD4+ T cell (Trm) development in mucosal tissues, and the optimization of protective T cell immunity in next generation COVID vaccines.
We are seeking a highly motivated, skilled, and organized research technician to assist in a diverse range of duties pertaining to nearly all operations of the lab.
Job responsibilities will include:
- general lab maintenance
- DNA plasmid cloning
- recombinant protein expression and purification
- minor oversight of mouse colony (no weaning or genotyping)
- basic mouse procedures (injections)
- mouse dissection and tissue preparation
- tissue culture
- advanced flow cytometry with peptide:MHC tetramer reagents
- in vitro assays including ELISpot, ELISA, and AIM (activation-induced marker)
- molecular assays including cDNA preparation for single cell RNA-seq analysis
The applicant must have a B.A., B.S, or M.S. in a science-related discipline, preferably with some background in immunology. Experience in mouse husbandry, tissue culture, flow cytometry, or computer programming is highly desired, but not required. The applicant must be well organized, technically proficient, critically minded, and able to work independently. Applicants must be US citizens or permanent residents (department will not sponsor visas).
Interested applicants should submit a resume, contact information for 2 references, and a short description of relevant work experience and long-term career goals.