Research Specialist 97597
Research Specialist
Multi-disciplinary lab seeks a motivated Research Specialist to carry out research into the pathological mechanisms of glaucoma in a team environment.
Specific Duties & Responsibilities
- Perform various glaucoma model and optic nerve ophthalmic surgeries and intraocular injections in mice and rats, preparing and dosing animals in drug studies, measuring intraocular pressure using rebound tonometry, using injectable or gas anesthetics via commercial rodent anesthesia machine in accordance with USDA and AALAC.
- Prepare and carry out perfusion fixation and micro-dissection of ocular tissues.
- Histological methods performed in this position include cryopreservation and epoxy preparation (embedding and sectioning) for immunofluorescent and/or morphologic studies of animal and human donor eye tissues.
- Management of multiple mouse lines, performing ear punches, breeding and tail PCF to genotype, maintaining database of mouse lines.
- Image capture and data analysis for morphological studies using analysis programs such as Metamorph, Imaris, and ImageJ.
- Confocal and or 2 photon imaging of immuno-labelled tissues.
- Execute proper disposal of biohazardous materials, maintain laboratory conditions and other duties as assigned by Lab Manager.
Minimum Qualifications
- Bachelor's Degree in Biology, Chemistry or related field.
- One year of laboratory experience performing relevant techniques.
- Master's Degree, with related graduate research, may substitute for experience to the extent permitted by the JHU equivalency formula.
- This position requires working with rodents on a frequent basis. Those candidates with pre-existing allergies to rodents or cats and/or have asthma will be unsuitable for this job.
Preferred Qualifications
- 1 year experience in a cell biology, molecular biology or neuroscience laboratory performing relevant techniques is desired.
- Histology, animal surgery and microdissection, immunofluorescence microscopy, and confocal microscopy desired.